GigaScience aims to revolutionize publishing by promoting reproducibility of analyses and data dissemination, organization, understanding, and use. As an open access and open-data journal, we publish ALL research objects (data, software tools and workflows) from ‘big data’ studies across the entire spectrum of life and biomedical sciences. These resources are managed using the FAIR Principles for scientific data management and stewardship that state that research data should be Findable, Accessible, Interoperable and Reusable. To achieve our goals, the journal has a novel publication format: one that links standard manuscript publication with an extensive database that hosts all associated data and provides data analysis tools and cloud-computing resources. GigaDB provides a direct link between the published manuscript and the relevant supporting data. We have also built GigaGalaxy, a Galaxy-based data analysis platform to host computational methods and workflows, maximizing use of the data, tools and workflows in our papers in a more accessible and reproducible environment. Our scope covers not just ‘omic’ type data and the fields of high-throughput biology currently serviced by large public repositories, but also the growing range of more difficult-to-access data, such as imaging, neuroscience, ecology, cohort data, systems biology and other new types of large-scale sharable data. All articles and content (including blogs and peer reviews) published by GigaScience are made freely and permanently accessible online immediately upon publication, without subscription charges or registration barriers. All software is published under Open Source Initiative (OSI)-approved open source licences, and supporting data presented under a public domain CC0 waiver. Further information about our open access policies and our article processing charges to support these efforts can be found here. This will be added to Biological Informatics Subject Tracer™. This will be added to Healthcare Resources Subject Tracer™. This will be added to Business Intelligence Resources Subject Tracer™. This will be added to Entrepreneurial Resources Subject Tracer™. This will be added to the tools section of Research Resources Subject Tracer™. This will be added to Reference Resources Subject Tracer™.